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Primary Antibodies
APOE Antibody
Background
APOE Antibody.
Additional Names
APOE, AD2, MGC1571, apoprotein, apolipoprotein E, apolipoprotein E3, Alzheimer disease 2 (APOE*E4-associated, late onset)
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Description
Left: Western blot analysis of APOE in human brain lysate (35μg protein in RIPA buffer) using APOE antibody (0.1μg/mL).
Below: Immunohistochemistry analysis of APOE in human liver using APOE antibody (2.5μg/mL).
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Other Product Images
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Source
APOE antibody was raised against a synthetic peptide of APOE.
Purification
APOE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
APOE antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx. 36kDa band observed in Human Brain lysates (calculated MW of 36.2kDa according to NP_000032.1). Recommended concentration: 0.1-0.3μg/ml. Immunohistochemistry: In paraffin embedded Human Liver shows extra-cellular staining between hepatocytes. Recommended concentration, 2-4μg/ml.
Tested Application
E, WB, IHC
Buffer
APOE antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-267P - APOE Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, Chm
Protein Accession Number
NP_000032.1
Short Description
APOE
This product belongs to the following categories:
Related Products
References- Dodart JC, Marr RA, Koistinaho M, Gregersen BM, Malkani S, Verma IM, Paul SM. Gene delivery of human apolipoprotein E alters brain A{beta} burden in a mouse model of Alzheimer's disease. Proc Natl Acad Sci U S A. 2005 Jan 25;102(4):1211-6. Epub 2005 Jan 18.
Datasheet 08-01W
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