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Primary Antibodies
APOC1 Antibody
Background
APOC1 Antibody.
Additional Names
APOC1, apolipoprotein C-I
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Description
Left: Immunohistochemistry staining of APOC1 in paraffin embedded human liver using APOC1 antibody at 3.8μg/mL.
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Source
APOC1 antibody was raised against a synthetic peptide of APOC1.
Purification
APOC1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
APOC1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Preliminary experiments gave bands at 30kDa and 37kDa band in Human Liver lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 9.3kDa according to NP_001636.1. The detected bands were successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded Human Liver shows spotty staining in the cytoplasm of hepatocytes. Recommended concentration, 3-5μg/ml.
Tested Application
E, IHC
Buffer
APOC1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-266P - APOC1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H
Protein Accession Number
NP_001636.1
Short Description
APOC1
This product belongs to the following categories:
Related Products
References- Berbee JF, van der Hoogt CC, Sundararaman D, Havekes LM, Rensen PC. Severe hypertriglyceridemia in human APOC1 transgenic mice is caused by apoC-I-induced inhibition of LPL. J Lipid Res. 2005 Feb;46(2):297-306. Epub 2004 Dec 1.
Datasheet 08-01W
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