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Primary Antibodies
APOBEC3G Antibody
Background
The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates (1). Similar to TRIM5α which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection (2,3) as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome (4,5). This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity (6).
Additional Names
APOBEC3G (NT), APOBEC3G
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Description
Left: Western blot analysis of APOBEC3G expression in Caco-2 cell lysate in the (A), absence and (B) presence of blocking peptide with APOBEC3G antibody at 5 μg/ml. .
Below: Immunohistochemical staining of human spleen using APOBEC3G antibody at 1 μg/ml.
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Other Product Images
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Source
APOBEC3G antibody was raised against a synthetic peptide corresponding to 15 amino acids near the amino-terminus of human APOBEC3G APOBEC3G antibody will also detect the APOBEC3F isoform.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
APOBEC3G antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human APOBEC3G (N-Terminus) Peptide (Cat. No. 3257P)
Application
APOBEC3G antibody can be used for detection of APOBEC3G by Western blot at 5 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3257P - APOBEC3G Peptide
Storage
APOBEC3G antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1223 - Caco-2 Cell Lysate
- Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity
H
Protein GI Number
13399304
Protein Accession Number
NP_068594
Short Description
(NT) an HIV restriction factor
This product belongs to the following categories:
Related Products
References- Jarmuz A, Chester A, Bayliss J, et al. An anthropoid-specific locus of Orphan C to U RNA-editing enzymes on chromosome 22. Genomics 2002; 79:285-96.
- Stremlau M, Owens CM, Perron MJ, et al. The cytoplasmic body component TRIM5α restricts HIV-1 infection in Old World monkeys. Nature 2004; 427:848-53.
- Bieniasz PD. Intrinsic immunity: a front-line defense against viral attack. Nat Immunol. 2004; 5:1109-15.
- Sheehy AM, Gaddis NC, Choi JD, et al. Isolation of a human gene that inhibits HIV-1 infection and is suppressed by the viral Vif protein. Nature 2002; 418:646-50.
Datasheet 08-02W
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