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Primary Antibodies
APOBEC3D Antibody
Background
APOBEC3D Antibody.
Additional Names
APOBEC3D, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D (putative), ARP6, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3D
Source
APOBEC3D antibody was raised against a synthetic peptide of APOBEC3D.
Purification
APOBEC3D antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
APOBEC3D antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 37kDa band in lysates of cell lines A549, HEK293, HepG2, K562 and U937 after 1μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 31.7kDa according to NP_689639.1. The 37kDa band was successfully blocked by incubation with the immunizing peptide.
Tested Application
E
Buffer
APOBEC3D antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-265P - APOBEC3D Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H
Protein Accession Number
NP_689639.1
Short Description
APOBEC3D
This product belongs to the following categories:
Related Products
References- Stenglein MD, Harris RS. APOBEC3B and APOBEC3F inhibit L1 retrotransposition by a DNA deamination-independent mechanism. J Biol Chem. 2006 Jun 23;281(25):16837-41. Epub 2006 Apr 28.
Datasheet 08-01W
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