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Primary Antibodies
APIP Antibody
Background
The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Recently, Cho et al (4) have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.
Additional Names
APIP, APIP2, CGI-29, DJ179L10.2, MMRP19
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Description
Left: Immunohistochemistry staining of APIP in kidney (formalin-fixed paraffin-embedded) using APIP antibody.
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Purification
Protein G Column
Clonality / Clone
This is a polyclonal antibody.
Isotype
IgG
Host
APIP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
APIP antibody was raised against a full-length His-tagged recombinant APIP protein. of APIP. (Human)
Application
APIP antibody can be used for detection of APIP by WB (1 - 2 μg/ml), IHC-parafin (10 μg/ml).
Tested Application
WB, IHC
Buffer
APIP antibody is supplied in PBS, 0.2% gelatin, 0.05% sodium azide.
Storage
APIP antibody can be stored at 4˚C for short term storage, for long term storage aliquot and store at -20˚C . Avoid freeze thaw cycles.
Species Reactivity
H
Formulation
Weight: 50 μg
Concentration: 0.5 mg/ml
Short Description
Likely Ortholog Of Mouse Monocyte Macrophage 19
This product belongs to the following categories:
Datasheet 09-01W
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