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Primary Antibodies
APH1A Antibody
Background
APH1A Antibody. This antibody is expected to recognize both reported isoforms (NP_001071096.1 and NP_057106.2).
Additional Names
APH1A, anterior pharynx defective 1 homolog A (C. elegans), 6530402N02Rik, APH-1A, CGI-78, anterior pharynx defective 1 homolog A
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Description
Left: Western blot analysis of APH1A in human cerebral cortex lysate (35μg protein in RIPA buffer) using APH1A antibody (0.05μg/mL).
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Source
APH1A antibody was raised against a synthetic peptide of APH1A.
Purification
APH1A antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
APH1A antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approx 26kDa band observed in human brain (cerebral cortex) lysates (calculated MW of 26.8kDa according to NP_057106.2). Recommended concentration: 0.05-0.1μg/ml.
Tested Application
E, WB
Buffer
APH1A antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-262P - APH1A Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H, M, R
Protein Accession Number
NP_001071096.1, NP_057106.2
Short Description
APH1A
This product belongs to the following categories:
Related Products
References- Spasic D, Raemaekers T, Dillen K, Declerck I, Baert V, Serneels L, Fullekrug J, Annaert W. Rer1p competes with APH-1 for binding to nicastrin and regulates gamma-secretase complex assembly in the early secretory pathway. J Cell Biol. 2007 Feb 26;176(5):629-40.
Datasheet 08-01W
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