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Primary Antibodies
APH1 Antibody
Background
APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the γsecretase complex which cleave the amyloid precursor protein (APP) at what are known as the γ and εsites and can lead to the accumulation of the Amyloid βpeptide Aβcleavage product that is associated with Alzheimer’s disease. APH1 exists in at least three distinct isoforms with APH1α as the principal isoform present in the γsecretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE.
Additional Names
APH1 (NT), Anterior pharynx defective 1, presenilin stabilization factor
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Description
Left: Western blot analysis of APH1 in RAW264.7 cell lysate with APH1 antibody at (A) 0.5, (B) 1 and (C) 2 μg/ml.
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Source
APH1 antibody was raised against a 13 amino acid peptide from near the amino terminus of human APH1a.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
APH1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
APH1 antibody can be used for detection of APH1 by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4001P - APH1 Peptide
Storage
APH1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1283 - RAW264.7 Cell Lysate
Species Reactivity
H, M
Protein GI Number
14250557
Protein Accession Number
AAH08732
Short Description
(NT) a component of the presenilin complex
This product belongs to the following categories:
Related Products
References- Goutte C, Tsunozaki M, Hale VA, et al. APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos. Proc. Natl. Acad. Sci. USA 2002; 99:775-9.
- Periz G and Fortini ME. Functional reconstitution of γsecretase through coordinated expression of presenilin, nicastrin, aph-1, and pen-2. J. Neurosci. Res. 2004; 77:309-22.
- Selkoe DJ. The cell biology of?βamyloid precursor protein and presenilin in Alzheimer’s disease. Trends Cell Biol. 1998; 8:447-53.
- Ma G, Li T, Price DL, et al. APH-1a is the principal mammalian aph-1 isoform present in g-secretase complexes during embryonic development. Neuro. Dis. 2005; 25:192-8.
Datasheet 08-02W
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