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Recombinant Proteins
APG5L Recombinant Protein
Background
Mammalian cells undergoing apoptosis express high levels of APG5L (or ASP) protein relatively late in the apoptotic process. APG5L protein contains a high number of acidic residues, a potential glycosylation site, and potential casein kinase II, protein kinase C, and tyrosine kinase sites.
FUNCTION: May play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.
PTM: Conjugated to ATG12; which is essential for autophagy, but is not required for association with isolation membrane.
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Description
SDS PAGE: Analysis of APG5L Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.
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Source
E. coli
Tested Application
E, WB, MS
Buffer
10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3, 10mM DTT
Storage
Store at -70˚C. As with any protein, exposing APG5L recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/aH
Protein GI Number
4757798
Protein Accession Number
NP_004840
Domain Information
aa. 1-275
Molecular Weight
31.6 kDa (Calculated)
Fusion Partner
T7 tag at N-terminus
Purity
95%
Short Description
APG5, autophagy 5-like, ASP, apoptosis specific protein
This product belongs to the following categories:
References- Grand, R. J. A.; Milner, A. E.; Mustoe, T.; Johnson, G. D.; Owen, D.; Grant, M. L.; Gregory, C. D. : A novel protein expressed in mammalian cells undergoing apoptosis. Exp. Cell Res. 218: 439-451, 1995.
Datasheet 0803-W
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