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Primary Antibodies
APEX1 Antibody
Background
APEX1 Antibody. NP_001632.2; NP_542379.1 and NP_542380.1 are varients that represent the same protein.
Additional Names
APEX, APEX1, APEX nuclease (multifunctional DNA repair enzyme), APE, APX, APE1, APEN, HAP1, REF1, REF-1, AP lyase, AP endonuclease class I, apurinic/apyrimidinic exonuclease, multifunctional DNA repair enzyme, DNA-(apurinic or apyrimidinic site) lyase, a
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Description
Left: Western blot analysis of APEX1 in A431 lysate (RIPA buffer, 30μg total protein per lane) using APEX1 antibody (0.5μg/mL).
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Source
APEX1 antibody was raised against a synthetic peptide near the N-terminus of APEX1.
Purification
APEX1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
APEX1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Western blot: Approx 35kDa band seen in A431 lysate. Recommended for use at 0.2-1μg/ml.
Tested Application
E, WB
Buffer
APEX1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-009P - APEX1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1202 - A431 Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_001632.2, NP_542379.1, NP_542380.1
Short Description
(NT) APE1 / APEX1
This product belongs to the following categories:
Related Products
References- Demple B, Herman T, Chen DS. Cloning and expression of APE, the cDNA encoding the major human apurinic endonuclease: definition of a family of DNA repair enzymes. Proc Natl Acad Sci U S A. 1991 Dec 15;88(24):11450-4.
Datasheet 08-02W
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