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Primary Antibodies
APBB1 Antibody
Background
APBB1 Antibody. This antibody is expected to recognize both reported isoforms (NP_001155.1 and NP_663722.1).
Additional Names
APBB1, amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65), FE65, MGC:9072, RIR, adaptor protein FE65a2, amyloid beta A4 precursor protein-binding, family B, member 1, stat-like protein
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Description
Left: Western blot analysis of APBB1 in NIH/3T3 lysate (35μg protein in RIPA buffer) using APBB1 antibody (0.1μg/mL).
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Source
APBB1 antibody was raised against a synthetic peptide of APBB1.
Purification
APBB1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
APBB1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:128,000. Approx 100kDa band observed in lysates of NIH/3T3 and HeLa (calculated MW of 77.2kDa according to NP_001155.1 and 77.0kDa according to NP_663722.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Zambrano et al, J Biol Chem. 1998 Aug 7;273(32):20128-33.; PMID: 9685356). Recommended concentration: 0.1-0.3μg/ml.
Tested Application
E, WB
Buffer
APBB1 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-261P - APBB1 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_001155.1, NP_663722.1
Short Description
APBB1 / FE65
This product belongs to the following categories:
Related Products
References- Hoe HS, Magill LA, Guenette S, Fu Z, Vicini S, Rebeck GW. FE65 Interaction with the apoE receptor ApoEr2. J Biol Chem. 2006 Apr 25; [Epub ahead of print]
Datasheet 08-01W
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