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Primary Antibodies
APAF1 Antibody
Background
Apoptotic protease activating factor 1. Apaf-1 is a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.
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Description
Apaf-1 IgY Antibody
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Location
The gene that codes for this protein is at chromosomal location 12q23.
Purification
Antigen affinity-purified
Clonality / Clone
This is a polyclonal antibody.
Host
APAF1 antibody was raised in chicken.
Please use anti-chicken secondary antibodies.
Immunogen
307-388
Application
Apaf-1 antibody can be used for the detection of Apaf-1 by Western blot, may also work for IHC and ICC.
Tested Application
WB
Buffer
Phosphate-Buffered Saline. No preservatives added.
Storage
APAF1 antibody can be stored at 4˚C for short term (weeks). Long term storage should be at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H, M
Protein GI Number
32483361
Protein Accession Number
NP_863658.1
Molecular Weight
141.8 kDa (calculated)
Short Description
Apoptotic protease activating factor 1
This product belongs to the following categories:
Related Products
References- Cecconi,F., Alvarez-Bolado,G., Meyer,B. I., Roth,K. A. and Gruss,P. Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 94 (6), 727-737 (1998),
Datasheet 0801-W
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