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Recombinant Proteins
AP2M1 Recombinant Protein
Background
AP2M1 is a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. This protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. It may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein.
FUNCTION: Component of the adaptor complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. AP50 is a subunit of the plasma membrane adaptor. The complex binds polyphosphoinositide-containing lipids.
SUBUNIT: Adaptor protein complex 2 (AP-2) is an heterotetramer composed of two large adaptins (α1A/AP2A1 or α1B/AP2A1 or α2/AP2A2 and β1/AP2B1), a medium adaptin (mu2/AP2M1) and a small adaptin (σ2long/AP2S1 or σ2short/AP2S1). Interacts with ATP6V1H.
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Description
SDS PAGE: Analysis of AP2M1 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.
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Source
E. coli
Tested Application
E, WB, MS
Buffer
10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3
Storage
Store at -70˚C. As with any protein, exposing AP2M1 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/aH
Protein GI Number
14917109
Protein Accession Number
NP_004059
Domain Information
aa. 1-435
Molecular Weight
50.0 kDa (Calculated)
Fusion Partner
T7 tag at N-terminus
Purity
95%
Short Description
Adaptor-related protein complex 2, clathrin-associated assembly adaptor protein
This product belongs to the following categories:
Related Products
References- Motley,A., Bright,N.A., Seaman,M.N. and Robinson,M.S. Clathrin-mediated endocytosis in AP-2-depleted cells. J. Cell Biol. 162 (5), 909-918 (2003)
Datasheet 0803-W
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