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Primary Antibodies
AP2M1 Antibody
Background
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family. The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP2M1 has also been shown to associate with the HIV-1 protein Nef, suggesting that Nef may use AP-2 complex to enhance the rate of endocytosis of both CD4 and class I MHC. AP2M1 may also play an important role in regulating the intracellular trafficking and function of cytotoxic T-lymphocyte associated (CTLA)-4 protein. At least two isoforms of AP2M1 are known to exist.
Additional Names
AP2M1, Adaptor-related protein complex 2 mu 1, AP-2 mu-1, mu2, AP50, CLAPM1
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Description
Left: Western blot analysis of AP2M1 in human kidney tissue lysate with AP2M1 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunohistochemistry of AP2M1 in mouse kidney tissue with AP2M1 antibody at 2.5 μg/ml.
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Other Product Images
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Source
AP2M1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human AP2M1.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
AP2M1 antibody was raised in chicken.
Please use anti-chicken secondary antibodies.
Application
AP2M1 antibody can be used for detection of AP2M1 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5161P - AP2M1 Peptide
Storage
AP2M1 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
14917109
Protein Accession Number
NP_004059
Short Description
Adaptor-related protein complex 2 mu 1
This product belongs to the following categories:
References- Robinson MS. Adaptable adaptors for coated vesicles. Trends Cell Biol. 2004; 14:167-74.
- Aguilar RC, Ohno H, Roche KW, et al. Functional domain mapping of the clathrin-associated adaptor medium chains mu1 and mu J. Biol. Chem. 1997; 272:27160-6.
- Liu Q, Feng Y, and Forgac M. Activity and in vitro reassembly of the coated vesicle (H+)-ATPase requires the 50-kDa subunit of the clathrin assembly complex AP-2. J. Biol. Chem. 1994; 269:31592-7.
- Craig HM, Reddy TR, Riggs NL, et al. Interactions of HIV-1 Nef with the m subunits of adaptor protein complexes 1, 2, and 3: role of the dileucine-based sorting motif. Virology 271:9-17.
Datasheet 09-01W
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