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Primary Antibodies
AOC3 Antibody
Background
AOC3 Antibody.
Additional Names
AOC3, amine oxidase, copper containing 3 (vascular adhesion protein 1), HPAO, SSAO, VAP-1, VAP1, amine oxidase, copper containing 3, copper amine oxidase, semicarbazide-sensitive amine oxidase, vascular adhesion protein 1
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Description
Left: Immunohistochemistry staining of AOC3 in paraffin embedded human liver using AOC3 antibody at 3.8μg/mL.
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Source
AOC3 antibody was raised against a synthetic peptide of AOC3.
Purification
AOC3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
AOC3 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Preliminary experiments gave an approx 55kDa band in Human Adipose and Lung lysates after 0.01μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 84.6kDa according to NP_003725.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded Human Liver shows membranous staining in sinusoid lining cells surrounding hepatocytes. Recommended concentration, 3-5μg/ml.
Tested Application
E, IHC
Buffer
AOC3 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-258P - AOC3 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H, M, R
Protein Accession Number
NP_003725.1
Short Description
AOC3
This product belongs to the following categories:
Related Products
References- Forster-Horvath C, Dome B, Paku S, Ladanyi A, Somlai B, Jalkanen S, Timar J. Loss of vascular adhesion protein-1 expression in intratumoral microvessels of human skin melanoma. Melanoma Res. 2004 Apr;14(2):135-40.
Datasheet 08-01W
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