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Primary Antibodies
ANILLIN Antibody
Background
ANILLIN Antibody.
Additional Names
ANLN, anillin, actin binding protein (scraps homolog, Drosophila), Scraps, ANILLIN, anillin (Drosophila Scraps homolog), actin binding protein
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Description
Left: Immunohistochemistry staining of ANILLIN in human kidney using AILLIN antibody at 10μg/ml.
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Source
ANILLIN antibody was raised against a synthetic peptide near the C-terminus of ANILLIN.
Purification
ANILLIN antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ANILLIN antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules. Recommended concentration, 3-10μg/ml.
Tested Application
E, IHC
Buffer
ANILLIN antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-253P - ANILLIN Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1305 - Human Kidney Tissue Lysate
Species Reactivity
H, M
Protein Accession Number
NP_061155.2
Short Description
(CT) ANILLIN / Scraps
This product belongs to the following categories:
Related Products
References- Oegema K, Savoian MS, Mitchison TJ, Field CM. Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis. J Cell Biol. 2000 Aug 7;150(3):539-52.
Datasheet 08-01W
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