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Catalog Number:

45-248


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REDI-Ladder™ Molecular Weight Marker


Antibody Production


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Primary Antibodies

AMID Antibody

Background

AMID Antibody.

Additional Names

AMID, PRG3, apoptosis-inducing factor (AIF)-like mitochondrion-associated inducer of death, 5430437E11Rik, p53-responsive gene 3, apoptosis-inducing factor (AIF)-homologous mitochondrion-associated inducer of death

Source

AMID antibody was raised against a synthetic peptide of AMID.

Purification

AMID antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality / Clone

This is a polyclonal antibody.

Host

AMID antibody was raised in goat.

Please use anti-goat secondary antibodies.

Application

Peptide ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Preliminary experiments gave an approx 48-50kDa band in human colon and duodenum lysates after 0.1μg/ml antibody staining.

Tested Application

E

Buffer

AMID antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Blocking Peptide

Cat. No. 45-248P - AMID Peptide

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Species Reactivity

H

Protein Accession Number

NP_116186

Short Description

AMID / PRG3

This product belongs to the following categories:

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References

  1. Ohiro Y, Garkavtsev I, Kobayashi S, Sreekumar KR, Nantz R, Higashikubo BT, Duffy SL, Higashikubo R, Usheva A, Gius D, Kley N, Horikoshi N. A novel p53-inducible apoptogenic gene, PRG3, encodes a homologue of the apoptosis-inducing factor (AIF). FEBS Lett. 2002 Jul 31;524(1-3):163-71.


Datasheet 08-01W

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