|
Primary Antibodies
AMACR Monoclonal Antibody
Background
AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.
Additional Names
RACE
|
Description
Left: Western blot analysis using AMACR monoclonal antibody against truncated AMACR - His recombinant protein and Jurkat cell lysate.
Below: Immunohistochemical analysis of paraffin - embedded human prostate adenocarcinoma tissue showing cytoplasma location using AMACR antibody with DAB staining.
|
|
Other Product Images
|
Clonality / Clone
This is a monoclonal antibody. Clone 2A10F3.
Isotype
IgG2b
Host
AMACR monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Purified truncated recombinant AMACR-His fusion protein expressed in E. Coli strain BL21 (DE3).
Application
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Tested Application
E, WB, IHC
Storage
AMACR monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
23600
Short Description
alpha-methylacyl-CoA racemase
This product belongs to the following categories:
Related Products
References- Chen Q. Watson JT. Marengo SR. et al. Cancer Lett. 2006, Dec 8, 244 (2):274-88.Epub 2006 Feb 23.
- Epstein JI. Herawi M. J Urol. 2006, Mar, 175 (3 Pt 1):820-34. Review.
Datasheet 0903-W
|
|
