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Primary Antibodies
ALOX5AP Antibody
Background
ALOX5AP Antibody.
Additional Names
ALOX5AP, FLAP, arachidonate 5-lipoxygenase-activating protein, MK-886-binding protein, five-lipoxygenase activating protein
Source
ALOX5AP antibody was raised against a synthetic peptide near the C-terminus of ALOX5AP.
Purification
ALOX5AP antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ALOX5AP antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 48kDa band in human brain and U937 lysates at 1ug/ml. Further, this band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 19kDda according to NP_001620. ALOX5AP antibody has been successfully used in WB assays with transfected HeLa cells (customer feedback, see Maier TJ et al., 2008).
Tested Application
E, WB
Buffer
ALOX5AP antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-241P - ALOX5AP Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H
Protein Accession Number
NP_001620
Short Description
(CT) FLAP / ALOX5AP
This product belongs to the following categories:
Related Products
References- Helgadottir A, Manolescu A, Thorleifsson G, Gretarsdottir S, Jonsdottir H, Thorsteinsdottir U, Samani NJ, Gudmundsson G, Grant SF, Thorgeirsson G, Sveinbjornsdottir S, Valdimarsson EM, Matthiasson SE, Johannsson H, Gudmundsdottir O, Gurney ME, Sainz J, Thorhallsdottir M, Andresdottir M, Frigge ML, Topol EJ, Kong A, Gudnason V, Hakonarson H, Gulcher JR, Stefansson K. The gene encoding 5-lipoxygenase activating protein confers risk of myocardial infarction and stroke. Nat Genet. 2004 Mar;36(3):233-9. Epub 2004 Feb 08.
Datasheet 08-01W
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