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Primary Antibodies
ALOX15 Antibody
Background
ALOX15 Antibody.
Additional Names
ALOX15, arachidonate 15-lipoxygenase, 15-LOX-1, 15-LOX2
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Description
Left: Western blot analysis of ALOX15 in HeLa lysate (35μg protein in RIPA buffer) using ALOX15 antibody (1μg/ml).
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Source
ALOX15 antibody was raised against a synthetic peptide of ALOX15.
Purification
ALOX15 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ALOX15 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration: 0.2-0.5μg/ml.
Tested Application
E, WB
Buffer
ALOX15 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-769P - ALOX15 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_001131.3
Short Description
ALOX15
This product belongs to the following categories:
Related Products
References- McDuffie M, Maybee NA, Keller SR, Stevens BK, Garmey JC, Morris MA, Kropf E, Rival C, Ma K, Carter JD, Tersey SA, Nunemaker CS, Nadler JL. Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes. Diabetes. 2008 Jan;57(1):199-208. Epub 2007 Oct 16.
Datasheet 09-01W
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