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Primary Antibodies
AKT3 Antibody
Background
AKT3 Antibody. This antibody is expected to recognize an epitope corresponding to aa 119-133 of both isoforms of human AKT3 protein. This antibody does not cross-react with human AKT1/2.
Additional Names
AKT3, PKBG, PRKBG, STK-2, RAC-gamma, RAC-PK-gamma, DKFZP434N0250, v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), protein kinase B gamma, serine threonine protein kinase, Akt-3, RAC-gamma serine/threonine protein kinase
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Description
Left: Western blot analysis of AKT3 in HepG2 lysate (35μg protein in RIPA buffer) using AKT3 antibody (1μg/mL).
Below: HEK293 overexpressing AKT3 with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with AKT3 antibody in the right panel (vector-only transfection in first and fourth lanes).
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Other Product Images
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Source
AKT3 antibody was raised against a synthetic peptide at an internal region of AKT3.
Purification
AKT3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
AKT3 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:16,000. Western Blot: A 50-55kDa band observed in human hepatoblastoma HepG2 lysates (calculated MW of 55.8kDa according to NP_005456 and 54.0kDa according to NP_859029). Recommended concentration: 1-3μg
Tested Application
E, WB
Buffer
AKT3 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-005P - AKT3 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity
H, M, R
Protein Accession Number
NP_005456, NP_859029
Short Description
(IN) AKT3
This product belongs to the following categories:
Related Products
References- Zinda MJ, Johnson MA, Paul JD, Horn C, Konicek BW, Lu ZH, Sandusky G, Thomas JE, Neubauer BL, Lai MT, Graff JR. AKT-1, -2, and -3 are expressed in both normal and tumor tissues of the lung, breast, prostate, and colon. Clin Cancer Res. 2001 Aug;7(8):2475-9.
Datasheet 09-01W
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