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Primary Antibodies
AKT1 Antibody
Background
AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Additional Names
AKT1,v-akt murine thymoma viral oncogene homolog 1, PKB, PRKBA, RAC, RAC-ALPHA
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Description
Left: Western blot analysis of AKT1 expression in fetal small intestine lysate using AKT1 antibody 0.25µg/ml.
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Purification
Rabbit Ig G is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
AKT1 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human AKT1.
Application
AKT1 antibody can be used for Western blot at a suggested dilution at 0.25µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Tested Application
WB
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
Antibody is lyophilized from PBS buffer with 2% sucrose. Add 50 µl of distilled water. Final antibody concentration is 1 mg/ml. For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
Positive Control
- Cat. No. XBL-10420 - Human Fetal Small Intestine Tissue Lysate
Species Reactivity
H, M, D
Protein Accession Number
NP_005154
Molecular Weight
56kDa
Short Description
Anti-AKT1
This product belongs to the following categories:
Related Products
References- Kim,H.A., et al., (2006) Exp. Mol. Pathol. 80 (2), 165-170
Datasheet 0802-W
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