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Primary Antibodies
AIFM3 Antibody
Background
Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Recently a protein similar to the apoptosis-inducing factor (AIF) was cloned and designated AIFL (also known as AIFM3). AIFM3 is expressed ubiquitously and is predominantly localized to the inner membranes of mitochondria. Unlike AIF, AIFM3 does not translocate to the nucleus upon induction of apoptosis. However, overexpression of AIFM3, like AIF, induced cytochrome c release from the mitochondria, cleavage of caspase 3, and ultimately apoptosis, indicating AIFM3 induces apoptosis through caspase activation. Multiple isoforms of AIFM3 are known to exist.
Additional Names
AIFM3 (IN), Apoptosis-inducing factor mitochondrion-associated 3, AIFL
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Description
Left: Western blot analysis of AIFM3 in human brain tissue lysate with AIFM3 antibody at (A) 1 and (B) 2 μg/ml.
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Source
AIFM3 antibody was raised against a 16 amino acid peptide from near the center of human AIFM3.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
AIFM3 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
AIFM3 antibody can be used for the detection of AIFM3 by Western blot at 1 – 2 μg/ml.
Tested Application
E, WB
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4541P - AIFM3 Peptide
Storage
AIFM3 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
74732608
Protein Accession Number
Q96NN9
Short Description
(IN) Apoptosis-inducing factor mitochondrion-associated 3
This product belongs to the following categories:
Related Products
References- Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther. 2004; 4:139-63
- Xie Q, Lin T, Zhang Y, et al. Molecular cloning and characterization of a human AIF-like gene with the ability to induce apoptosis. J. Biol. Chem. 2005; 280:19673-81.
Datasheet 08-02W
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