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Primary Antibodies
AIF Antibody
Background
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and widely expressed (2).
Additional Names
AIF (IN), AIF
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Description
Left: Western blot analysis of AIF in K562 cell lysate (A), rat heart (B), and mouse heart (C) tissue lysates with AIF antibody (IN) at 1 μg/ml.
Below: Immunohistochemistry of AIF in human retina with AIF antibody at 10 μg/ml.
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Other Product Images
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Source
AIF antibody was raised against a peptide corresponding to amino acids 517 to 531 of human AIF. This sequence is identical to those of mouse and rat AIF.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
AIF antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human AIF (Intermediate Domain) Peptide (Cat. No. 2267P)
Application
AIF antibody can be used for detection of AIF by Western blot at 0.25 to 1 μg/ml. A 67 kDa band should be detected.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2267P - AIF Peptide
Storage
AIF antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
- Cat. No. 1461 - Rat Heart Tissue Lysate
- Cat. No. 1401 - Mouse Heart Tissue Lysate
Species Reactivity
H, M, R
Protein GI Number
13431764
Protein Accession Number
O95831
Short Description
(IN) Apoptosis Inducing Factor
This product belongs to the following categories:
Related Products
References- Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8
- Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-6
- Daugas E, Susin SA, Zamzami N, et al. Mitochondrio-nuclear translocation of AIF in apoptosis and necrosis. FASEB J 2000;14:729-39 (WD0500)
Datasheet 08-02W
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