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Primary Antibodies
AIF Antibody
Background
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and widely expressed (2).
Additional Names
AIF (NT), AIF
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Description
Left: Western blot analysis of AIF in K562 cell lysate with AIF antibody at 1 μg/ml.
Below: Immunocytochemistry of AIF??in Jurkat cells with AIF antibody at 2 μg/ml.
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Other Product Images
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Source
AIF antibody was raised against a peptide corresponding to amino acids near the amino terminus of mature human AIF.
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
AIF antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human AIF (N-Terminus) Peptide (Cat. No. 2239P)
Application
AIF antibody can be used for detection of AIF by Western blot at 0.25 to 1 μg/ml. A 67 kDa band should be detected.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2239P - AIF Peptide
Storage
AIF antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein GI Number
13431764
Protein Accession Number
O95831
Short Description
(NT) Apoptosis Inducing Factor
This product belongs to the following categories:
Related Products
References- Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999;401:127-8
- Susin SA, Lorenzo HK, Zamzami N, et al. Molecular characterization of mitochondrial apoptosis-inducing factor. Nature 1999;397:441-6 (WD0500)
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Datasheet 08-02W
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