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Primary Antibodies
AID Antibody
Background
Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines (1). It is necessary for somatic hypermutation and class switch recombination in B cells (2), but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms (2,3). Although it is structurally and functionally similar to the APOBEC proteins (reviewed in 4), it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G (5).
Additional Names
AID
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Description
Left: Western blot analysis of AID in Ramos whole cell lysate with AID antibody at 2 μg/ml in either the (A) absence or (B) presence of blocking peptide.
Below: Immunocytochemistry of AID in Ramos cells with Ramos antibody at 10 μg/ml.
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Other Product Images
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Source
AID antibody was raised against a peptide corresponding to 14 amino acids near the carboxy-terminus of human AID.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
AID antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human AID Peptide (Cat. No. 3091P)
Application
AID antibody can be used for detection of AID by Western blot at 1 - 2 μg/ml. Whole cell lysate from Ramos cells can be used as positive control. Anti-AID is human and mouse reactive.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3091P - AID Peptide
Storage
AID antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1225 - Ramos Cell Lysate
Species Reactivity
H, M
Protein GI Number
22297218
Protein Accession Number
AAM95402
Short Description
activation induced cytidine deaminase
This product belongs to the following categories:
Related Products
References- Muramatsu M, Sankaranand VS, Anant S, et al. Specific expression of activation-induced cytidine deaminase (AID), a novel member of the RNA-editing deaminase family in germinal center B cells. J. Biol. Chem. 1999; 274:18470-6.
- Muramatsu M, Kinoshita K, Fagarasan S, et al. Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme. Cell 2000; 102:553-63.
- Greeve J, Philipsen A, Krause K, et al. Expression of activation-induced cytidine deaminase in human B-cell Hodgkin lymphomas. Blood 2003; 101:3574-80.
- Cascalho M. Advantages and disadvantages of cytidine deamination. J. Immunol. 2004; 172:6513-8.
Datasheet 08-02W
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