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Primary Antibodies
AES Antibody
Background
Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the α5β1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the α5β1 integrin (4).
Additional Names
AES (CT), Amino-terminal enhancer of split / GRG / ESP1 / TLE5
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Description
Left: Western blot analysis of AES in 293 cell lysate with AES antibody at (A) 1, (B) 2 and (C) 4 μg/ml.
Below: Immunocytochemistry of AES in HeLa cells with AES antibody at 10 μg/ml.
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Other Product Images
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Source
AES antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human AES.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
AES antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (C-Terminus) Peptide (Cat. No. 3607P)
Application
AES antibody can be used for the detection of AES by Western blot at 1 - 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3607P - AES Peptide
Storage
AES antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1210 - 293 Cell Lysate
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R
Protein GI Number
39812019
Protein Accession Number
NP_945320
Short Description
(CT) Amino-terminal enhancer of split
This product belongs to the following categories:
Related Products
References- Martin SS and Vuori K. Regulation of Bcl-2 proteins during anoikis and amorphosis. Biochim Biophys Acta. 2004; 1692:145-57.
- Miyasaka H, Choudhury BK, Hou WE, et al. Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein. Eur. J. Biochem. 1993; 216:343-52.
- Chen G and Courey AJ. Groucho/TLE family proteins and transcriptional repression. Gene 2000; 249:1-16.
- Jan Y, Matter M, Pai J-t, et al. A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and groucho/TLE corepressors. Cell 2004; 116:751-762.
Datasheet 08-02W
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