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Primary Antibodies
AEBP1 Antibody
Background
The adipocyte enhancer binding protein 1 (AEBP1) is a transcriptional repressor with carboxypeptidase (CP) activity. This protein binds to a regulatory sequence, adipocyte enhancer 1 (AE-1), located in the proximal promoter region of the adipose P2 (aP2) gene, which encodes the adipocyte fatty-acid binding protein. It is characterized as a member of the regulatory B-like CP family. This protein seems to be activated by a novel mechanism, whereby the direct binding of DNA enhances its protease activity. AEBP1 may play a role in differentiated vascular smooth muscle cells.
Additional Names
ACLP
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Description
Left: Western blot analysis of AEBP1 in HepG2 cell lysate using anti-AEBP1.
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Purification
Rabbit IgG is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
AEBP1 antibody was raised in rabbit.
Please use anti-rabbit IgG secondary antibodies.
Immunogen
AEBP1 antibody was produced in rabbits immunized with a synthetic peptide corresponding to a region of human AEBP1.
Application
AEBP1 antibody can be used for Western blot at a suggested dilution of 0.2-5.0 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti Rabbit IgG should be diluted in 1: 50,000 - 100,015 as secondary antibody. The protein size is 1158 amino acids.
Tested Application
WB, IHC
Buffer
Antibody is supplied in PBS buffer with 2% sucrose. Product may ship lyophilized. To reconstitute add 100μl of distilled water making the final concentration 1 mg/ml.
Storage
AEBP1 antibody is supplied in PBS buffer containing 0.02% sodium azide. For longer periods of storage, store at -20ºC. Avoid repeat freeze-thaw cycle.
Positive Control
- Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_001120
Molecular Weight
131kDa
Short Description
Anti-Adipocyte Enhancer Binding Protein 1 (AEBP1)
This product belongs to the following categories:
Related Products
References- Layne,M.D.,et al.,(1998) J.Biol.Chem.273(25),15654-15660
Datasheet 0802-W
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