|
Primary Antibodies
ADRA2B Antibody
Background
FUNCTION: α-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The rank order of potency for agonists of this receptor is clonidine > norepinephrine > epinephrine = oxymetazoline > dopamine > p-tyramine = phenylephrine > serotonin > p-synephrine / p-octopamine. For antagonists, the rank order is yohimbine > chlorpromazine > phentolamine > mianserine > spiperone > prazosin > alprenolol > propanolol > pindolol.
SUMMARY: α-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: α2A, α2B, and α2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. This protein is the α2B subtype, which was observed to associate with eIF-2B, a guanine nucleotide exchange protein that functions in regulation of translation. A polymorphic variant of the α2B subtype, which lacks 3 glutamic acids from a glutamic acid repeat element, was identified to have decreased G protein-coupled receptor kinase-mediated phosphorylation and desensitization; this polymorphic form is also associated with reduced basal metabolic rate in obese subjects and may therefore contribute to the pathogenesis of obesity. This protein contains no introns in either its coding or untranslated sequences.
Additional Names
Alpha2B adrenergic receptor, Alpha-2B adrenoceptor, Alpha-2B adrenoreceptor, Alpha-2 adrenergic receptor subtype C2
|
Description
Left: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
|
Location
The gene that codes for this protein is at chromosomal location 2p13-q13.
Purification
Immunoaffinity Purified
Clonality / Clone
This is a polyclonal antibody.
Host
ADRA2B antibody was raised in chicken.
Please use anti-chicken secondary antibodies.
Immunogen
200-299
Application
α-2B adrenergic receptor antibody can be used for the detection of α-2B adrenergic receptor by Western Blot.
Tested Application
WB
Buffer
Phosphate-Buffered Saline. No preservatives added.
Storage
ADRA2B antibody can be stored at 4˚C for short term (weeks). Long term storage should be at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H
Protein GI Number
4501965
Protein Accession Number
NP_000673.1
Molecular Weight
50 kDa (calculated)
Short Description
a-2B adrenergic receptor
This product belongs to the following categories:
Datasheet 0801-W
|
|
