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Primary Antibodies
ADH7 Antibody
Background
ADH7 Antibody.
Additional Names
ADH7, alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide, ADH-4, alcohol dehydrogenase-7, class IV alcohol dehydrogenase 7 mu or sigma subunit, gastric alcohol dehydrogenase.
Source
ADH7 antibody was raised against a synthetic peptide of ADH7.
Purification
ADH7 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ADH7 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 26kDa band in lysates of human duodenum and liver and of rat stomach after 0.2μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.5kDa according to NP_000664.2. The 26kDa band was successfully blocked by incubation with the immunizing peptide.
Tested Application
E
Buffer
ADH7 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-208P - ADH7 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H, M
Protein Accession Number
NP_000664.2
Short Description
ADH7
This product belongs to the following categories:
Related Products
References- Zgombic-Knight M, Foglio MH, Duester G. Genomic structure and expression of the ADH7 gene encoding human class IV alcohol dehydrogenase, the form most efficient for retinol metabolism in vitro. J Biol Chem. 1995 Mar 3;270(9):4305-11.
Datasheet 08-01W
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