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Primary Antibodies
ADAP Antibody
Background
The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappaB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.
Additional Names
ADAP (NT), Adhesion and degranulation adapter protein, FYN binding protein, FYB-120/130, SLAP-130
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Description
Left: Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 μg/ml.
Below: Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 μg/ml.
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Other Product Images
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Source
ADAP antibody was raised against a 17 amino acid peptide from near the amino terminus of human ADAP.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ADAP antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application
ADAP antibody can be used for detection of ADAP by Western blot at 0.5 – 1 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4279P - ADAP Peptide
Storage
ADAP antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1204 - K562 Cell Lysate
Species Reactivity
H
Protein GI Number
42476118
Protein Accession Number
NP_001456
Short Description
(NT) Adhesion and degranulation adapter protein
This product belongs to the following categories:
Related Products
References- Griffiths EK, Krawczyk C, Kong YY, et al. Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap. Science 2001; 293:2260-3.
- Rawlings DJ, Sommer K, and Moreno-Garcia ME. The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes. Nat. Rev. Immunol. 2006; 6:799-812.
- Medeiros RB, Burbach BJ, Mueller KL, et al. Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1. Science 2007; 316:754-8.
- Mueller KL, Thomas MS, Burbach BJ, et al. Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival. J. Immunol. 2007; 179:3559-69.
Datasheet 08-02W
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