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Primary Antibodies
ADAM33 Antibody
Background
ADAM33 Antibody. This antibody is expected to recognize isoform alpha (NP_079496) and not isoform beta (NP_694882).
Additional Names
ADAM33, a disintegrin and metalloproteinase domain 33, HGNC:15478, DJ964F7.1, DKFZp434K0521, FLJ35308, FLJ36751, MGC71889, a disintegrin and metalloprotease 33, disintegrin and reprolysin metalloproteinase family protein, metalloprotease disintegrin
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Description
Left: Western blot analysis of ADAM33 in human prostate lysate (35μg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide using ADAM33 antibody (0.3μg/mL).
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Source
ADAM33 antibody was raised against a synthetic peptide of ADAM33.
Purification
ADAM33 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ADAM33 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:64,000. Western Blot: experiments gave bands at approx 80-90kDa and strong ladder from 26 to 38kDa in human prostate lysates after 0.3μg/ml antibody staining. The observation of multiple bands and the 38 kDa band correspond to earlier findings in literature with different antibodies (Powell et al, Am J Respir Cell Mol Biol. 2004 Jul;31(1):13-21; PMID: 14742294). This protein has a calculated MW of 87.7kDa according to NP_079496.1. Recommended concentration: 0.1-0.3μg/ml.
Tested Application
E, WB
Buffer
ADAM33 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-204P - ADAM33 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1312 - Human Prostate Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_079496.1
Short Description
ADAM33
This product belongs to the following categories:
Related Products
References- Blakey J, Halapi E, Bjornsdottir US, Wheatley A, Kristinsson S, Upmanyu R, Stefansson K, Hakonarson H, Hall IP. Contribution of ADAM33 polymorphisms to the population risk of asthma. Thorax. 2005 Apr;60(4):274-6.
Datasheet 08-01W
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