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Primary Antibodies
ADAM17 Antibody
Background
ADAM17 Antibody. This antibody is expected to recognize isoform 1 (represented by NP_003174) and not isoform 2 (represented by NP_068604).
Additional Names
ADAM17, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), CSVP, TACE, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM metallopeptidase domain 17, TNF-alpha converting enzyme, a disintegrin and metalloproteinas
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Description
Left: Western blot analysis of ADAM17 in HeLa lysate (RIPA buffer, 35μg total protein per lane) using ADAM17 antibody (0.2μg/mL).
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Source
ADAM17 antibody was raised against a synthetic peptide near the C-terminus of ADAM17.
Purification
ADAM17 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ADAM17 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approx 100kDa band observed in human testis lysates (calculated MW of 93.0kDa according to NP_003174.3). Recommended concentration: 0.1-0.3μg/ml.
Tested Application
E, WB
Buffer
ADAM17 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-002P - ADAM17 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H, M, R, HM
Protein Accession Number
NP_003174.3
Short Description
(CT) ADAM17 / TACE
This product belongs to the following categories:
Related Products
References- Black RA, Rauch CT, Kozlosky CJ, Peschon JJ, Slack JL, Wolfson MF, Castner BJ, Stocking KL, Reddy P, Srinivasan S, Nelson N, Boiani N, Schooley KA, Gerhart M, Davis R, Fitzner JN, Johnson RS, Paxton RJ, March CJ, Cerretti DP A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature. 1997 Feb 20;385(6618):729-33
Datasheet 08-02W
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