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Primary Antibodies
ADAM12 Antibody
Background
ADAM12 Antibody. This antibody is expected to recognize both the longer membrane-bound form of human ADAM12 (NP_003465) and the shorter soluble ADAM12 splice isoform (NP_067673). This antibody does not cross-react with other ADAMS.
Additional Names
ADAM12, MCMP, MLTN, MLTNA, MCMPMltna, a disintegrin and metalloproteinase domain 12 (meltrin alpha), meltrin alpha, A disintegrin and metalloproteinase domain 12 (Meltrin-alpha, mouse, homolog of)
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Description
Left: Western blot analysis of ADAM12 in human heart lysate (RIPA buffer, 30μg total protein per lane) using ADAM12 antibody (0.1μg/mL).
Below: Immunohistochemistry staining of ADAM12 in human prostate using ADAM12 antibody (3.8μg/ml).
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Other Product Images
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Source
ADAM12 antibody was raised against a synthetic peptide near the N-terminus of ADAM12.
Purification
ADAM12 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ADAM12 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: Approx 90kDa band observed in human heart lysates (calculated MW of 99.6kDa according to NP_003465 and 80.5kDa according to NP_067673). Recommended concentration: 0.1-0.3μg/ml. Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasm staining of a group of secretory epithelial cells. Recommended concentration, 3-5μg/ml.
Tested Application
E, WB, IHC
Buffer
ADAM12 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-001P - ADAM12 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_003465, NP_067673
Short Description
(NT) ADAM12
This product belongs to the following categories:
Related Products
References- Galliano MF, Huet C, Frygelius J, Polgren A, Wewer UM, Engvall E. Binding of ADAM12, a marker of skeletal muscle regeneration, to the muscle-specific actin-binding protein, alpha -actinin-2, is required for myoblast fusion. J Biol Chem. 2000 May 5;275(18):13933-9.
Datasheet 08-02W
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