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Primary Antibodies
ADAM10 Antibody
Background
Proinflammatory cytokine tumor necrosis factor-α (TNF-α) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-α, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-α, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).
Additional Names
ADAM10 (CT), KUZ
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Description
Left: Western blot analysis of ADAM10 in Jurkat whole cell lysate with ADAM10 antibody at (A) 1 and (B) 2 μg/ml.
Below: Immunocytochemistry staining of K562 cells using ADAM10 antibody at 2 μg/ml.
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Other Product Images
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Source
ADAM10 antibody was raised against a peptide corresponding to amino acids 732 to 748 of human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid (2,4).
Purification
Ion exchange chromatography purified
Clonality / Clone
This is a polyclonal antibody.
Host
ADAM10 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human ADAM10 (C-Terminus) Peptide (Cat. No. 2051P)
Application
ADAM10 can be used for detection of ADAM10 by Western blot at 1:500 to 1:2000 dilution. An approximately 85 kDa band can be detected, which may represent precursor. This polyclonal antibody can also detect ADAM10 by immunohistochemistry at 2 μg/ml.
Tested Application
E, WB, ICC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 2051P - ADAM10 Peptide
Storage
ADAM10 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate
Species Reactivity
H
Protein GI Number
4557251
Protein Accession Number
NP_001101
Short Description
(CT) TNFα converting enzyme
This product belongs to the following categories:
Related Products
References- Rosendahl MS, Ko SC, Long DL, et al. Identification and characterization of a pro-tumor necrosis factor-α-processing enzyme from the ADAM family of zinc metalloproteases. J Biol Chem 1997;272:24588-93
- Pan D, Rubin GM. Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis. Cell 1997;90:271-80
- Qi H, Rand MD, Wu X, Sestan N, Wang W, Rakic P, Xu T, Artavanis-Tsakonas S. Processing of the notch ligand delta by the metalloprotease Kuzbanian. Science 1999;283:91-4
Datasheet 08-02W
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