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Primary Antibodies
ADA Antibody
Background
ADA is an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Additional Names
ADA
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Description
Left: Western blot analysis of ADA in fetal muscle lysate using ADA antibody at 1 μg/ml (12% gel).
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Purification
Antibody is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
ADA antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ADA.
Application
ADA antibody can be used for detection of ADA by ELISA at 1:62500. ADA antibody can be used for detection of ADA by western blot at 1 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store ADA antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H, M
Protein Accession Number
NP_000013
Molecular Weight
41kDa
Short Description
ADA (adenosine deaminase)
This product belongs to the following categories:
References- Cassani,B., (2008) Blood 111 (8), 4209-4219.
Datasheet 10-01W
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