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Primary Antibodies
ACVR2B Antibody
Background
Activin receptors are all transmembrane proteins, composed of a ligand-binding extracellular domain with cysteine-rich region, a transmembrane domain, and a cytoplasmic domain with predicted serine/threonine specificity. Type I receptors are essential for signaling; and type II receptors are required for binding ligands and for expression of type I receptors. Type I and II receptors form a stable complex after ligand binding, resulting in phosphorylation of type I receptors by type II receptors. Type II receptors are considered to be constitutively active kinases. ACVR2B is activin A type IIB receptor, which displays a 3- to 4-fold higher affinity for the ligand than activin A type II receptor.
Additional Names
ACVR2B
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Description
Left: Western blot analysis of ACVR2B in HepG2 Cell Lysate using ACVR2B antibody at 0.25 μg/ml (12% gel). Marker in lane A.
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Purification
Antibody is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
ACVR2B antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ACVR2B.
Application
ACVR2B antibody can be used for detection of ACVR2B by ELISA at 1:312500. ACVR2B antibody can be used for detection of ACVR2B by western blot at 0.25 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store ACVR2B antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H, D
Protein Accession Number
AAH96245
Molecular Weight
33kDa, 58kDa
Short Description
ACVR2B (activin A receptor, type IIB)
This product belongs to the following categories:
Datasheet 10-01W
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