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Recombinant Proteins
ACTL6A Recombinant Protein
Background
The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. This gene encodes a 53 kDa subunit protein of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila; the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix.
FUNCTION: Involved in transcriptional activation and repression of select genes by chromatin remodelling (alteration of DNA-nucleosome topology). Required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
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Description
SDS PAGE: Analysis of ACTL6A Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.
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Source
E. coli
Tested Application
E, WB, MS
Buffer
10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3
Storage
Store at -70˚C. As with any protein, exposing ACTL6A recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/aH
Protein GI Number
4757718
Protein Accession Number
NP_004292
Domain Information
aa. 1-429
Molecular Weight
49.3 kDa (Calculated)
Fusion Partner
T7 tag at N-terminus
Purity
95%
Short Description
ARP, BAF complex, BRG1-associated factor, actin-related protein, hArpN beta, BAF53a isoform 1
This product belongs to the following categories:
References- Zhao, K.; Wang, W.; Rando, O. J.; Xue, Y.; Swiderek, K.; Kuo, A.; Crabtree, G. R. : Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling. Cell 95: 625-636, 1998.
Datasheet 0803-W
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