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Recombinant Proteins
ACTG1 Recombinant Protein
Background
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, α, β and γ have been identified. The α actins are found in muscle tissues and are a major constituent of the contractile apparatus. The β and γ actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, γ 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells.
FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
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Description
SDS PAGE: Analysis of ACTG1 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.
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Source
E. coli
Tested Application
E, WB, MS
Buffer
10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3
Storage
Store at -70˚C. As with any protein, exposing ACTG1 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/aH
Protein GI Number
4501887
Protein Accession Number
NP_001605
Domain Information
aa. 1-375
Molecular Weight
41.8 kDa (Calculated)
Fusion Partner
T7 tag at N-terminus
Purity
>95%
Short Description
actin, actin gamma 1 propeptide, cytoskeletal gamma-actin, actin cytoplasmic 2
This product belongs to the following categories:
References- Cheng,C. and Sharp,P.A.: RNA polymerase II accumulation in the promoter-proximal region of the dihydrofolate reductase and gamma-actin genes. Mol. Cell. Biol. 23 (6), 1961-1967 (2003)
Datasheet 0803-W
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