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Primary Antibodies
ACTB Antibody
Background
FUNCTION: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
SUBUNIT: Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.
SIMILARITY: Belongs to the actin family.
Additional Names
Nonmuscle actin (b and gamma), Beta-actin
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Description
Western Blot: 293 cell lysate as test antigen. Affinity-purified IgY dilution: 1:1000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
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Location
The gene that codes for this protein is at chromosomal location 7p15-p12.
Purification
Immunoaffinity Purified
Clonality / Clone
This is a polyclonal antibody.
Host
ACTB antibody was raised in chicken.
Please use anti-chicken secondary antibodies.
Immunogen
1-375
Application
Non-muscle actin (β and γ) antibody can be used for the detection of Non-muscle actin (β and γ) by Western Blot.
Tested Application
WB
Buffer
Phosphate-Buffered Saline. No preservatives added.
Storage
ACTB antibody can be stored at 4˚C for short term (weeks). Long term storage should be at -20˚C. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity
H, M, R, B, C, Rb
Protein GI Number
4501885
Protein Accession Number
NP_001092.1
Molecular Weight
41.7 kDa (calculated)
Short Description
Non-muscle actin (b and g), b-actin
This product belongs to the following categories:
Datasheet 0801-W
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