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Primary Antibodies
ACOX2 Antibody
Background
ACOX2 Antibody.
Additional Names
ACOX2, acyl-Coenzyme A oxidase 2, branched chain, BCOX, BRCACOX, BRCOX, THCCox, Peroxisomal branched chain acyl-CoA oxidase, THCA-CoA oxidase, Trihydroxycoprostanoyl-CoA oxidase
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Description
Left: Western blot analysis of ACOX2 in human liver lysate (35μg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide using ACOX2 antibody (0.5μg/mL).
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Source
ACOX2 antibody was raised against a synthetic peptide near the C-terminus of ACOX2.
Purification
ACOX2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ACOX2 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Approx 75kDa band observed in human liver lysates (calculated MW of 76.8kDa according to NP_003491.1). Recommended concentration: 0.5-1.5μg/ml. An additional band of 37kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Tested Application
E, WB
Buffer
ACOX2 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-194P - ACOX2 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1304 - Human Liver Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_003491.1
Short Description
(CT) ACOX2
This product belongs to the following categories:
Related Products
References- Baumgart E, Vanhooren JC, Fransen M, Marynen P, Puype M, Vandekerckhove J, Leunissen JA, Fahimi HD, Mannaerts GP, van Veldhoven PP. Molecular characterization of the human peroxisomal branched-chain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, and evidence for the absence of the protein in Zellweger syndrome. Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13748-53.
Datasheet 08-01W
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