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Primary Antibodies
ACHE Antibody
Background
ACHE Antibody. This antibody is expected to recognize isoform NP_000656 only (the ubiquitously expressed, hydrophillic form).
Additional Names
ACHE, YT, acetylcholinesterase (YT blood group)
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Description
Left: Western blot analysis of ACHE in human cerebellum lysate (35μg protein in RIPA buffer) using ACHE antibody (1μg/mL).
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Source
ACHE antibody was raised against a synthetic peptide of ACHE.
Purification
ACHE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ACHE antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:2000. Western Blot: Approx 70kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 67.8kDa according to NP_000656.1). Recommended concentration: 1-3μg/ml.
Tested Application
E, WB
Buffer
ACHE antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-193P - ACHE Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. XBL-10110 - Human Hippocampus Tissue Lysate
Species Reactivity
H, M, R, C, Rb
Protein Accession Number
NP_000656.1
Short Description
ACHE
This product belongs to the following categories:
Related Products
References- Cottingham MG, Voskuil JL, Vaux DJ. The intact human acetylcholinesterase C-terminal oligomerization domain is alpha-helical in situ and in isolation, but a shorter fragment forms beta-sheet-rich amyloid fibrils and protofibrillar oligomers. Biochemistry. 2003 Sep 16;42(36):10863-73.
Datasheet 08-01W
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