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Primary Antibodies
ACE2 Antibody
Background
Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology (1-2). In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus (3). The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function (4).
Additional Names
ACE2 (CT), ACE2
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Description
Left: Western blot analysis of ACE2 in human kidney lysate with ACE2 antibody at 0.5 (lane A), 1 (lane B), and 2 (lane C) μg/ml, respectively.
Below: Immunohistochemical staining of human kidney tissue using ACE2 antibody at 2 μg/ml.
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Other Product Images
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Source
ACE2 antibody was raised against a synthetic peptide corresponding to amino acids near the C-terminus of human ACE2.
Purification
Affinity chromatography purified via peptide column
Clonality / Clone
This is a polyclonal antibody.
Host
ACE2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Human ACE2 (C-Terminus) Peptide (Cat. No. 3217P)
Application
ACE2 antibody can be used for the detection of ACE2 by Western blot at 0.5 to 2 μg/ml. A 90 kDa band can be detected. Anti-ACE2 has no cross response to ACE1.
Tested Application
E, WB, IHC
Buffer
Antibody is supplied in PBS containing 0.02% sodium azide.
Storage
ACE2 antibody can be stored at 4˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1305 - Human Kidney Tissue Lysate
- Cat. No. 1313 - Human Testis Tissue Lysate
Species Reactivity
H
Protein GI Number
11225609
Protein Accession Number
NP_068576
This product belongs to the following categories:
Related Products
References- Donoghue M, Hsieh F, Baronas E, et al. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotension I to angiotension 1-9. Circ. Res. 2000;87:1-9.
- Tipnis SR, Hooper NM, Hyde R, et al. A human homolog of angiotensin-converting enzyme. Cloning and functional expression as a captopril-insensitive carboxypeptidase. J Biol. Chem. 2000;275:33238-43.
- Li W, Moore MJ, Vasileva N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature 2003;426:450-4.
Datasheet 08-02W
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