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Recombinant Proteins
ACAT2 Recombinant Protein
Background
Acetyl-Coenzyme A acetyltransferase 2 is an enzyme involved in lipid metabolism. Reported patients with ACAT2 deficiency have shown severe mental retardation and hypotonus. The ACAT2 gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation.
CATALYTIC ACTIVITY: 2 acetyl-CoA = CoA + acetoacetyl-CoA.
SUBUNIT: Homotetramer.
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Description
SDS PAGE: Analysis of ACAT2 Recombinant Protein. 4-20% SDS gradient gel. Coomassie blue staining.
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Source
E. coli
Tested Application
E, WB, MS
Buffer
10 mM Tris, pH 8.0, 0.1% Triton X-100, 0.002% NaN3
Storage
Store at -70˚C. As with any protein, exposing ACAT2 recombinant protein to repeated freeze/thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Species Reactivity
n/aH
Protein GI Number
5174389
Protein Accession Number
NP_005882
Domain Information
aa. 1-397
Molecular Weight
41.3 kDa (Calculated)
Fusion Partner
T7 tag at N-terminus
Purity
>95%
Short Description
acetyl-Coenzyme A acetyltransferase 2, acetoacetyl Coenzyme A thiolase
This product belongs to the following categories:
References- Masuno,M., Fukao,T., Song,X.Q., Yamaguchi,S., Orii,T., Kondo,N., Imaizumi,K. and Kuroki,Y.: Assignment of the human cytosolic acetoacetyl-coenzyme A thiolase (ACAT2) gene to chromosome 6q25.3-q26. Genomics 36 (1), 217-218 (1996)
Datasheet 0803-W
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