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Primary Antibodies
ACADM Antibody
Background
ACADM Antibody.
Additional Names
ACADM, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain, RP4-682C21.1, ACAD1, MCAD, MCADH, medium-chain acyl-CoA dehydrogenase
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Description
Left: Western blot analysis of ACADM in human heart lysate (RIPA buffer, 35μg total protein per lane) using ACADM antibody (0.05μg/mL).
Below: Immunocytochemistry of ACADM in paraffin embedded human liver using ACADM antibody (3.8μg/mL).
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Other Product Images
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Source
ACADM antibody was raised against a synthetic peptide of ACADM.
Purification
ACADM antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ACADM antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 45kDa band observed in Human Kidney and Heart lysates (calculated MW of 46.6kDa according to NP_000007.1). Recommended for use at 0.01-0.1μg/ml. Immunohistochemistry: In paraffin embedded Human Liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5μg/ml.
Tested Application
E, WB, IHC
Buffer
ACADM antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-190P - ACADM Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1301 - Human Heart Tissue Lysate
Species Reactivity
H
Protein Accession Number
NP_000007.1
Short Description
ACADM
This product belongs to the following categories:
Related Products
References- Matsubara Y, Kraus JP, Yang-Feng TL, Francke U, Rosenberg LE, Tanaka K. Molecular cloning of cDNAs encoding rat and human medium-chain acyl-CoA dehydrogenase and assignment of the gene to human chromosome 1. Proc Natl Acad Sci U S A. 1986 Sep;83(17):6543-7.
Datasheet 08-01W
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