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Primary Antibodies
ABL2 Monoclonal Antibody
Background
ABL2(ARG, Abelson-related gene) is a cytoplasmic tyrosine kinase which is closely related to but distinct from ABL1. The similarity of the proteins includes the tyrosine kinase domains and extends amino-terminal to include the SH2 and SH3 domains. ABL2 is expressed in both normal and tumor cells. The ABL2 gene product is expressed as two variants bearing different amino termini, both approximately 12-kb in length. c-Abl shows both cytoplasmic and nuclear localization?c-Abl is involved in two different chromosomal translocations present in human leukemias, which generate Bcr-Abl and TEL-Abl.
Additional Names
ARG, ABLL, FLJ22224, FLJ31718, FLJ41441
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Description
Left: Western blot analysis using ABL2 monoclonal antibody against truncated ABL2 recombinant protein.
Below: Immunofluorescense analysis of ehtanol - fixed Hela cells showing cytoplasma staining using ABL2 antibody with IgG - FITC.
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Other Product Images
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Clonality / Clone
This is a monoclonal antibody. Clone 1H1B11.
Isotype
IgG1
Host
ABL2 monoclonal antibody was raised in mouse.
Please use anti-mouse secondary antibodies.
Immunogen
Ni-NTA purified truncated recombinant ABL2 expressed in E. Coli strain BL21 (DE3).
Application
Dilution
Western Blot:1:200 - 1:1,000
IF:1:100 - 1:500
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Tested Application
E, WB, IF
Storage
ABL2 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
- Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity
H
Formulation
Ascitic fluid containing 0.03% sodium azide.
Purified antibody available at no extra cost.
Protein GI Number
27
Short Description
ARG, Abelson-related gene
This product belongs to the following categories:
Related Products
References- Yoshimi I, Takashi I, Tsuneyuki O, et al. Blood. 2000; 95(6): 2126-2131.
- Scheijen, B. and Griffin, J.D. Oncogene. 2002); 21£º3314-33.
Datasheet 0903-W
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