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Primary Antibodies
ABCC4 Antibody
Background
ABCC4 Antibody. This antibody is expected to recognize isoform 1 (NP_005836.2) only.
Additional Names
ABCC4, MRP4, MOATB, MOAT-B, EST170205, ATP-binding cassette, sub-family C (CFTR/MRP), member 4, canalicular multispecific organic anion transporter (ABC superfamily), Multidrug Resistance Protein 4
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Description
Left: Western blot analysis of ABCC4 in rat renal cortex membranes (20μg total protein per lane) using ABCC4 antibody (0.5μg/mL).
Below: ABCC4 staining of paraffin embedded human prostate using ABCC4 antibody at 2.5μg/mL.
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Other Product Images
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Source
ABCC4 antibody was raised against a synthetic peptide of ABCC4.
Purification
ABCC4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ABCC4 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approx 170kDa band observed in rat renal cortex membranes (calculated MW of 149kDa according to rat NP_596902.1 and 150kDa according to human NP_005836.2). Data kindly provided by Dr. Antje Steinbach, Ernst-Moritz-Arndt University of Greifswald, Germany. Recommended concentration: 1-3μg/ml.
Tested Application
E, WB, IHC
Buffer
ABCC4 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-181P - ABCC4 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1312 - Human Prostate Tissue Lysate
Species Reactivity
H, M, R
Protein Accession Number
NP_005836.2
Short Description
ABCC4 / MRP4
This product belongs to the following categories:
Related Products
References- Schuetz JD, Connelly MC, Sun D, Paibir SG, Flynn PM, Srinivas RV, Kumar A, Fridland A. MRP4: A previously unidentified factor in resistance to nucleoside-based antiviral drugs. Nat Med. 1999 Sep;5(9):1048-51.
Datasheet 08-01W
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