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Primary Antibodies
ABCB5 Antibody
Background
ABCB5 Antibody.
Additional Names
ATP-binding cassette, sub-family B (MDR/TAP), member 5 , ABCB5alpha, ABCB5beta, EST422562, ATP-binding cassette, sub-family B, member 5N: P-glycoprotein ABCB5
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Description
Left: Western blot analysis of ABCB5 in A431 cell lysate (35μg protein in RIPA buffer) using ABCB5 antibody (0.03μg/mL).
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Source
ABCB5 antibody was raised against a synthetic peptide of ABCB5.
Purification
ABCB5 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ABCB5 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:128,000. Western Blot: Approx 80kDa band observed in lysates of A431 (calculated MW of 89.8kDa according to NP_848654.3). Recommended concentration: 0.03-0.1μg/ml.
Tested Application
E, WB
Buffer
ABCB5 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 46-620P - ABCB5 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Positive Control
- Cat. No. 1202 - A431 Cell Lysate
Species Reactivity
H
Protein Accession Number
NP_848654.3
Short Description
ABCB5
This product belongs to the following categories:
Related Products
References- Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH. ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma. Cancer Res. 2005 May 15;65(10):4320-33.
Datasheet 09-01W
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