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Primary Antibodies
ABCB10 Antibody
Background
ABCB10 Antibody.
Additional Names
ABCB10, ATP-binding cassette, sub-family B (MDR/TAP), member 10, EST20237, M-ABC2, MTABC2, ATP-binding cassette, sub-family B, member 10, ABC-me
Source
ABCB10 antibody was raised against a synthetic peptide of ABCB10.
Purification
ABCB10 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality / Clone
This is a polyclonal antibody.
Host
ABCB10 antibody was raised in goat.
Please use anti-goat secondary antibodies.
Application
Peptide ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave bands at approx. 90kDa and 50kDa in human bone marrow lysates after 0.05μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 79.1kDa according to NP_036221.2 and given the expected mature size of ~65kDa according to Graf et al. (PMID: 15215243). Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
Tested Application
E
Buffer
ABCB10 antibody is supplied as 0.1mg of purified antibody, 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 45-179P - ABCB10 Peptide
Storage
Aliquot and store at -20°C. Minimize freezing and thawing.
Species Reactivity
H, M, R
Protein Accession Number
NP_036221.2
Short Description
ABCB10
This product belongs to the following categories:
Related Products
References- Graf SA, Haigh SE, Corson ED, Shirihai OS. Targeting, import, and dimerization of a mammalian mitochondrial ATP binding cassette (ABC) transporter, ABCB10 (ABC-me). J Biol Chem. 2004 Oct 8;279(41):42954-63
Datasheet 08-01W
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