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Primary Antibodies
ABAT Antibody
Background
4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities.4-aminobutyrate aminotransferase (ABAT) is responsible for catabolism of gamma-aminobutyric acid (GABA), an important, mostly inhibitory neurotransmitter in the central nervous system, into succinic semialdehyde. The active enzyme is a homodimer of 50-kD subunits complexed to pyridoxal-5-phosphate. The protein sequence is over 95% similar to the pig protein. GABA is estimated to be present in nearly one-third of human synapses. ABAT in liver and brain is controlled by 2 codominant alleles with a frequency in a Caucasian population of 0.56 and 0.44. The ABAT deficiency phenotype includes psychomotor retardation, hypotonia, hyperreflexia, lethargy, refractory seizures, and EEG abnormalities. Multiple alternatively spliced transcript variants encoding the same protein isoform have been found for this gene.
Additional Names
ABAT, GABA-AT, GABAT, NPD009
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Description
Left: Western blot analysis of ABAT in placenta lysate using ABAT antibody at 1 μg/ml (10-20% gel).
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Purification
Antibody is purified by peptide affinity chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
ABAT antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ABAT.
Application
ABAT antibody can be used for detection of ABAT by ELISA at 1:12500. ABAT antibody can be used for detection of ABAT by western blot at 1 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store ABAT antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H, M, R
Protein Accession Number
NP_065737
Molecular Weight
55kDa
Short Description
ABAT (4-aminobutyrate aminotransferase)
This product belongs to the following categories:
References- Inada,T., (2008) Pharmacogenet. Genomics 18 (4), 317-323.
Datasheet 10-01W
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