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Primary Antibodies
AATF Antibody
Background
The AATF is a protein that was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. AATF contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of AATF interfered with MAP3K12 induced apoptosis.The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.
Additional Names
AATF
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Description
Left: Western blot analysis of AATF in Daudi Cell Lysate using AATF antibody at 5 μg/ml (12% gel). Marker in lane A.
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Purification
Antibody is purified by protein A chromatography method.
Clonality / Clone
This is a polyclonal antibody.
Host
AATF antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AATF.
Application
AATF antibody can be used for detection of AATF by ELISA at 1:312500. AATF antibody can be used for detection of AATF by western blot at 5.0 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For longer periods of storage, store AATF antibody at -20˚C. Avoid repeat freeze-thaw cycles.
Species Reactivity
H
Protein Accession Number
NP_036270
Molecular Weight
63kDa
Short Description
AATF (apoptosis antagonizing transcription factor)
This product belongs to the following categories:
References- Lehner,B. (2004) Genome Res. 14 (7), 1315-1323.
Datasheet 10-01W
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